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Intended use
This immunoassay kit allows for the specific measurement of Human Orexin A concentrations in cell culture supernates, serum and plasma.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Orexin A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Orexin A present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Orexin A is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Orexin A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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